Anti-SYNPO2 Antibody Picoband™

Product Name

Anti-SYNPO2 Antibody Picoband™

View all Synpo2 Antibodies

SKU/Catalog Number

A07616-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SYNPO2 Antibody Picoband™ catalog # A05559-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SYNPO2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07616-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human SYNPO2 recombinant protein (Position: L104-R1009). Human SYNPO2 shares 79.6% and 78.6% amino acid (aa) sequence identity with mouse and rat SYNPO2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A07616-1 is reactive to Synpo2 in Human

Applications

A07616-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

160 kDa

Calculated molecular weight

116.527kDa

Background of Synpo2

Myopodin protein, also called Synaptopodin-2 is a protein that in humans is encoded by the SYNPO2 gene. Myopodin is expressed in cardiac, smooth muscle and skeletal muscle, and localizes to Z-disc structures.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SYNPO2 using anti-SYNPO2 antibody (A07616-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNPO2 antigen affinity purified polyclonal antibody (Catalog # A07616-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNPO2 at approximately 160 kDa. The expected band size for SYNPO2 is at 118 kDa.

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Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-SYNPO2 antibody (A07616-1).
Overlay histogram showing SH-SY5Y cells stained with A07616-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYNPO2 Antibody (A07616-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Synpo2 (Source: Uniprot.org, NCBI)

Alternative Names

synaptopodin-2; synaptopodin 2

For more info on Synpo2, check out the Synpo2 Infographic

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In this infographic, you will see the following information for Synpo2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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